Effective September 1, 2015; Banks in India will remain closed on the 2nd and 4th Saturdays of each month. U.S. visa applicants will not be able to pay the visa application fee on these days, even using the online National Electronic Funds Transfer (NEFT) facility. However, the Banks will work full day on all odd Saturdays (1st, 3rd and 5th). U.S. visa applicants should plan accordingly, especially if they have an urgent travel requirement.
This update can also be found here.